the

Löffler

lab

2008

  1. Fletcher, K. E., K. M. Ritalahti, B. K. Amos, K. D. Pennell, and F. E. Löffler. 2008. Resolution of culture Clostridium bifermentans DPH-1 into two populations: A Clostridium sp. and tetrachloroethene (PCE) dechlorinating Desulfitobacterium hafniense strain JH1. Appl. Environ. Microbiol. In review.

  2. Amos, B. A., K. M. Ritalahti, C. Cruz-Garcia, E. Padilla-Crespo, and F. E. Löffler. 2008. Oxygen effect on Dehalococcoides viability and biomarker quantification. Environ. Sci. Technol. 42:5718-5726.    pdf

  3. Amos, B. A., E. J. Suchomel, K. D. Pennell, and F. E. Löffler. 2008. Correlating microbial activity and distribution with enhanced contaminant dissolution from a NAPL source zone. Water Research. 42:2963-2974.     pdf

  4. Thomas, S. H., R. D. Wagner, A. K. Arakaki, J. Skolnick, J. R. Kirby, L. J. Shimkets, R. A. Sanford, and F. E. Löffler. 2008. The mosaic genome of Anaeromyxobacter dehalogenans strain 2CP-C suggests an aerobic common ancestor to the delta-proteobacteria. PLoS ONE 3(5): e2103. doi:10.1371. PMCID: PMC2330069     pdf

  5. Bradley, P. M., F. H. Chapelle, and F. E. Löffler. 2008. Anoxic mineralization: environmental reality or laboratory artifact? Ground Water Monitoring and Remediation. 28:47-49.    pdf


2007

  1. Sanford, R. A., Q. Wu, Q., Y. Sung, S. H. Thomas, B. K. Amos, E. K. Prince, and F. E. Löffler. 2007. Hexavalent uranium supports growth of Anaeromyxobacter dehalogenans and Geobacter spp. with lower than predicted biomass yields. Environ. Microbiol. 9:2885-2893.  PubMed: 17922770     pdf

  2. Amos, B. K., Y. Sung, K. E. Fletcher, T. J. Gentry, W.-M. Wu, C. S. Criddle, J. Zhou, and F. E. Löffler. 2007. Detection and quantification of Geobacter lovleyi strain SZ: implications for bioremediation at tetrachloroethene- (PCE-) and uranium-impacted sites. Appl. Environ. Microbiol. 73:6898-6904. PubMed: 17827319     pdf

  3. Bedard, D. L., K. M. Ritalahti and F. E. Löffler. 2007. Dehalococcoides in a sediment-free, mixed culture metabolically dechlorinate the commercial polychlorinated biphenyl mixture Aroclor 1260. Appl. Environ. Microbiol. 73:2513-2521.   PubMed: 17308182    pdf

  4. Daprato, R. C., F. E. Löffler, and J. B. Hughes. 2007. Comparative analysis of three tetrachloroethene to ethene halorespiring consortia suggests functional redundancy. Environ. Sci. Technol. 41:2261-2269.   PubMed: 17438773    pdf

  5. Amos B. K., R. C. Daprato, J. B. Hughes, Kurt D. Pennell, and F. E. Löffler. 2007. Effect of the nonionic surfactant Tween 80 on microbial reductive dechlorination of chloroethenes. Environ. Sci. Technol. 41:1710-1716.   PubMed: 17396664    pdf

  6. Amos, B. K., J. A. Christ, L. M. Abriola, K. D. Pennell, and F. E Löffler. 2006. Experimental evaluation and mathematical modeling of microbially-enhanced tetrachloroethene (PCE) dissolution. Environ. Sci. Technol. 41:963-970.   PubMed: 17328210   pdf

  7. Krajmalnik-Brown, Y. Sung, R., K. M. Ritalahti, F. Michael Saunders, and F. E. Löffler. 2007. Environmental distribution of the trichloroethene reductive dehalogenase gene (tceA) suggests lateral gene transfer among Dehalococcoides. FEMS Microbiol. Ecol. 59:206-214.   PubMed: 17233752    pdf


2006

  1. Löffler, F. E. 2006. Bakterieller abbau von vinylchlorid. LaborPraxis, Juli/August 2006.   pdf

  2. Löffler, F. E., and E. A. Edwards. 2006. Harnessing microbial activities for environmental cleanup. Curr. Opin. Biotechnol. 17:274-284.   PubMed: 16697178    pdf

  3. Okutman-Tas, D. I. N. Thomson, F. E. Löffler, and S. G. Pavlostathis. 2006. Kinetics of the microbial reductive dechlorination of pentachloroaniline. Environ. Sci. Technol. 40:4467-4472. PubMed: 16903287     pdf

  4. Wu, Q., R. A. Sanford, and F. E. Löffler. 2006. Uranium (VI) reduction by Anaeromyxobacter dehalogenans strain 2CP-C. Appl. Environ. Microbiol. 72:3608-3614.   PubMed: 16672509    pdf

  5. Rittmann, B. E, M. Hauser, F. E. Löffler, N. G. Love, G. Muyzer, S. Okabe, D. B. Oerther, J. Peccia, L. Raskin, and M. Wagner. 2006. A vista for microbial ecology and environmental biotechnology. Environ. Sci. Technol. 40:1096-1103.   PubMed: 16572761    pdf

  6. Sung, Y, K. M. Ritalahti, R. P. Apkarian, and F. E. Löffler. 2006. Quantitative PCR confirms purity of strain GT, a novel trichloroethene (TCE)-to-ethene-respiring Dehalococcoides isolate. Appl. Environ. Microbiol. 72:1980-1987.   PubMed: 16517646    pdf

  7. Ritalahti, K. M., K. K. Amos, Y. Sung, Q. Wu, S. S. Koenigsberg, and F. E. Löffler. 2006. Quantitative PCR targeting 16S rRNA and reductive dehalogenase genes simultaneously monitors multiple Dehalococcoides strains. Appl. Environ. Microbiol. 72:2765-2774.   PubMed: 16597981    pdf

  8. Sung, Y., K. E. Fletcher, K. M. Ritalahti, R. P. Apkarian, N. Ramos-Hernández, R. A. Sanford, N. M. Mesbah, and F. E. Löffler. 2006. Geobacter lovleyi Strain SZ sp. nov., a novel metal-reducing and tetrachloroethene (PCE)-dechlorinating bacterium. Appl. Environ. Microbiol. 72:2775-2782.  PubMed: 16597982    pdf

  9. Tront, J. M., B. K. Amos, F. E. Löffler, and F. M. Saunders. 2006. Activity of Desulfitobacterium sp. strain Viet1 demonstrates bioavailability of 2,4-dichlorophenol previously sequestered by the aquatic plant Lemna minor. Environ. Sci. Technol. 40:529-535.   PubMed: 16468399    pdf


2005

  1. Löffler, F. E., R. A. Sanford, and K. M. Ritalahti. 2005. Enrichment, cultivation, and detection of reductively dechlorinating bacteria.  Methods Enzymol. Vol. 397:77-111.  PubMed: 16260286     pdf

  2. Löffler, F. E., and R. A. Sanford. 2005. Analysis of trace hydrogen metabolism.  Methods Enzymol. Vol. 397:222-237.  PubMed: 16260294     pdf

  3. Waller, A. S., R. Krajmalnik-Brown, F. E. Löffler, and E. A. Edwards. 2005. Multiple reductive-dehalogenase-homologous genes are simultaneously transcribed during dechlorination by Dehalococcoides-containing cultures. Appl. Environ. Microbiol. 71:8257-8264.   PubMed: 16332811    pdf

  4. White, D. C., R. Geyer, A. D. Peacock, D. B. Hedrick, S. S. Koenigsberg, Y. Sung, J. He, and F. E. Löffler. 2005. Phospholipid furan fatty acids and ubiquinone-8: Possible free radical protective lipid biomarkers in Dehalococcoides. Appl. Environ. Microbiol. 71:8426-8433.   PubMed: 16332831    pdf

  5. He, J., Y. Sung, R. Krajmalnik Brown, K. M. Ritalahti, and F. E. Löffler. 2005. Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe. Environ. Microbiol. 7:1442-1450.   PubMed: 16104866    pdf

  6. Ritalahti, K. M., F. E. Löffler, E. R. Rasch, and S. S. Koenigsberg. 2005. Bioaugmentation for chlorinated solvent detoxification: Bioaugmentation and molecular diagnostics in the bioremediation of chlorinated ethene-contaminated sites. Industrial Biotechnology, 1:114-118.    pdf

  7. Christ, J. A., C. A. Ramsburg, L. M. Abriola, K. D. Pennell, and F. E. Löffler. 2005. Coupling aggressive mass removal with microbial reductive dechlorination for remediation of DNAPL source zones – a review and assessment. Environ. Health Perspec. 113:465-477.    PubMed: 15811838    pdf


2004

  1. Ramsburg, C. A., L. M. Abriola, K. D. Pennell, F. E. Löffler, M. Gamache, B. K. Amos, and E. A. Petrovskis. 2004. Stimulated microbial reductive dechlorination following surfactant treatment at the Bachman Road Site. Environ. Sci. Technol. 38:5902-5914.   PubMed: 15573588    pdf

  2. Krajmalnik-Brown, R., T. Hölscher, I. N. Thomson, F. M. Saunders, K. M. Ritalahti, and F. E. Löffler. 2004. Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp. strain BAV1. Appl. Environ. Microbiol. 70:6347-6351.   PubMed: 15466590    pdf

  3. Hölscher, T., R. Krajmalnik-Brown, K. M. Ritalahti, F. von Wintzingerode, H. Görisch, F. E. Löffler, and L. Adrian. 2004. Multiple non-identical putative reductive dehalogenase genes are common in Dehalococcoides. Appl. Environ. Microbiol. 70:5290-5297.    PubMed: 15345412    pdf

  4. Griffin, B. M., J. M. Tiedje, and F. E. Löffler. 2004. Anaerobic microbial reductive dechlorination of tetrachloroethene (PCE) to predominately trans-1,2-dichloroethene. Environ. Sci. Technol. 38:4300-4303.   PubMed: 15382856    pdf

  5. Ritalahti, K. M., and F. E. Löffler. 2004. Populations implicated in the anaerobic reductive dechlorination of 1,2-dichloropropane in highly enriched bacterial communities. Appl. Environ. Microbiol. 70:4088-4095.   PubMed: 15240287    pdf

  6. Singh, H., F. E. Löffler, and B. Z. Fathepure. 2004. Aerobic biodegradation of vinyl chloride by a highly enriched mixed culture. Biodegradation 15:197-204.    PubMed: 15228077    pdf


2003

  1. He, J., K. M. Ritalahti, K.-L. Yang, S. S. Koenigsberg, and F. E. Löffler. 2003. Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium. Nature 424:62-65.   PubMed: 12840758    pdf

  2. Major, D., E. Edwards, P. McCarty, J. Gossett, E. Hendrickson, F. Löffler, S. Zinder, D. Ellis, J. Vidumsky, M. Harkness, G. Klecka, and E. Cox. 2003. Discussion of environment vs. bacteria or let’s play, ‘name that bacteria’. Ground Water Monitoring & Remediation, 23:32-38.   pdf

  3. Sung, Y, K. M. Ritalahti, R. A. Sanford, J. W. Urbance, S. J. Flynn, J. M. Tiedje, and F. E. Löffler. 2003. Characterization of two tetrachloroethene (PCE)-reducing, acetate-oxidizing anaerobic bacteria, and their description as Desulfuromonas michiganensis sp. nov. Appl. Environ. Microbiol. 69:2964-2974.   PubMed: 12732573    pdf

  4. He, J., K. M. Ritalahti, M. R. Aiello, and F. E. Löffler. 2003. Complete detoxification of vinyl chloride by an anaerobic enrichment culture and identification of the reductively dechlorinating population as a Dehalococcoides species. Appl. Environ. Microbiol. 69:996-1003.    PubMed: 12571022    pdf

  5. Lendvay, J. M., F. E. Löffler, M. Dollhopf, M. R. Aiello, G. Daniels, B. Z. Fathepure, M. Gebhard, R. Heine, R. Helton, J. Shi, R. Krajmalnik-Brown, C. L. Major, Jr., M. J. Barcelona, E. Petrovskis, R. Hickey, J. M. Tiedje, and P. Adriaens. 2003. Bioreactive barriers: a comparison of bioaugmentation and biostimulation for chlorinated solvent remediation. Environ. Sci. Technol. 37:1422-1431.    pdf

  6. Gee, W. A., K. M. Ritalahti, W. D. Hunt, and F. E. Löffler. 2003. QCM viscometer for bioremediation and microbial activity monitoring. IEEE Sensors Journal, 3:304-309.    pdf

  7. Löffler, F. E., J. R. Cole, K. M. Ritalahti, and J. M. Tiedje. 2003. Diversity of dechlorinating bacteria, p. 53-87. In M. M. Häggblom and I. D. Bossert (eds.), Dehalogenation: microbial processes and environmental applications. Kluwer Academic, New York.     pdf


2002-1991

  1. He, J. Y. Sung, M. E. Dollhopf, B. Z. Fathepure, J. M. Tiedje, and F. E. Löffler. 2002. Acetate versus hydrogen as direct electron donors to stimulate the microbial reductive dechlorination process at chloroethene-contaminated sites. Environ. Sci. Technol. 36:3945-3952.    PubMed: 12269747    pdf

  2. Tesoriero, A. J., F. E. Löffler, and H. Liebscher. 2001. The fate and origin of fumigant-related compounds in an unconfined shallow aquifer. Environ. Sci. Technol. 35:455-461.   PubMed: 11351714    pdf

  3. Löffler, F. E. and K. M. Ritalahti. 2001. 16S rDNA-based tools identify Dehalococcoides species in many reductively-dechlorinating enrichment cultures, p. 53-68. In M. A. Kornmüller (ed.), Anaerobic dehalogenation. SFB 193 Biological Waste Water Remediation, Technical University Berlin, Germany.   pdf

  4. Löffler, F. E., Q. Sun, J. Li, and J. M. Tiedje. 2000. 16S rRNA Gene-based detection of tetrachloroethene (PCE)-dechlorinating Desulfuromonas and Dehalococcoides species. Appl. Environ. Microbiol. 66:1369-1374.   PubMed: 10742213    pdf

  5. Flynn, S. J., Löffler, F. E., and J. M. Tiedje. 2000. Microbial community changes associated with a shift from reductive dechlorination of PCE to reductive dechlorination of cis-DCE and VC. Environ. Sci. Technol. 34:1056-1061.   Abstract    pdf

  6. Löffler, F. E., J. M. Tiedje, and R. A. Sanford. 1999. The fraction of electrons consumed in electron acceptor reduction (fe) and hydrogen thresholds as indicators of halorespiratory physiology. Appl. Environ. Microbiol. 65:4049-4056.   PubMed: 10473415    pdf

  7. Wischnak, C., F. E. Löffler, J. Li, J. W. Urbance, and R. Müller. 1998. Isolation and characterization of a Pseudomonas species that degrades medium length a,w-dichloroalkanes. Appl. Environ. Microbiol. 64:3507-3511.    PubMed: 9726906    pdf

  8. Löffler, F. E., J. E. Champine, K. M. Ritalahti, S. J. Sprague, and J. M. Tiedje. 1997. Complete reductive dechlorination of 1,2-dichloropropane by anaerobic bacteria. Appl. Environ. Microbiol. 63:2870-2875.   PubMed: 16535654    pdf

  9. Löffler, F. E., K. M. Ritalahti, and J. M. Tiedje. 1997. Dechlorination of chloroethenes is inhibited by 2-bromoethanesulfonate in the absence of methanogens. Appl. Environ. Microbiol. 63:4982-4985.   PubMed: 16535759    pdf

  10. Löffler, F. E., R. A. Sanford, and J. M. Tiedje. 1996. Initial characterization of a reductive dehalo-genase from Desulfitobacterium chlororespirans Co23. Appl. Environ. Microbiol. 62:3809-3813.   PubMed: 16535425    pdf

  11. Sanford, R. A., J. R. Cole, F. E. Löffler, and J. M. Tiedje. 1996. Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate. Appl. Environ. Microbiol. 62:3800-3808.   PubMed: 8837437    pdf

  12. Löffler, F., F. Lingens, and R. Müller. 1995. Dehalogenation of 4-chlorobenzoate: Characterization of 4-chlorobenzoyl-coenzyme A dehalogenase from Pseudomonas sp. CBS3. Biodegradation 6:203-212.   PubMed: 7579994    pdf

  13. Löffler, F., R. Müller, and F. Lingens. 1992. Purification and characterization of 4-halobenzoate-coenzyme A ligase from Pseudomonas sp. CBS3. Biol. Chem. Hoppe-Seyler 373:1001-1007.   PubMed: 1418673    pdf

  14. Löffler, F., and R. Müller. 1991. Identification of 4-chlorobenzoyl-coenzyme A as intermediate in the dehalogenation catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3. FEBS Lett. 290:224-226.   PubMed: 1915880    pdf

  15. Löffler, F., R. Müller, and F. Lingens. 1991. Dehalogenation of 4-chlorobenzoate by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3: an ATP/coenzyme A dependent reaction. Biochem. Biophys. Res. Commun. 176:1106-1111.   PubMed: 2039495    pdf

  16. Elsner, A., F. Löffler, K. Miyashita, R. Müller, and F. Lingens. 1991. Resolution of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 into three components. Appl. Environ. Microbiol. 57:324-326.   PubMed: 2036019    pdf


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publications

I don’t really understand what I do.  The thing I do understand is that I have to do it all the time.

Nick Cave